Imaging microglia in live brain slices and slice cultures.
نویسندگان
چکیده
INTRODUCTIONThis protocol describes methods for fluorescence labeling and time-lapse confocal imaging of microglia in acutely prepared tissue slices from developing and adult animals, and to slice cultures derived from early post-natal day 7 (<PND7) animals. The techniques permit real-time analyses of microglial structure and dynamic remodeling in live rodent brain tissues.
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عنوان ژورنال:
- CSH protocols
دوره 2007 شماره
صفحات -
تاریخ انتشار 2007